Difference between revisions of "Part:BBa K3002126"
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− | This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a> | + | This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>), the His-tag a href="https://parts.igem.org/Part:BBa_K3002028">BBa_K3002028</a>)and the coding sequence of the Wildtype-PETase (<a href="https://parts.igem.org/Part:BBa_K3002004">BBa_K3002004</a>) plus the MoClo connectors for positions B2-B3 (<a href="https://parts.igem.org/Part:BBa_K3002003">BBa_K3002003</a>), B4-B5 (<a href="https://parts.igem.org/Part:BBa_K3002004">BBa_K3002004</a>)and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002005">BBa_K3002005</a>). |
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Revision as of 13:32, 21 October 2019
Level 1 Wildtype-PETase + His
This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), the His-tag a href="https://parts.igem.org/Part:BBa_K3002028">BBa_K3002028)and the coding sequence of the Wildtype-PETase (BBa_K3002004) plus the MoClo connectors for positions B2-B3 (BBa_K3002003), B4-B5 (BBa_K3002004)and B5-B6 (BBa_K3002005).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1002
Illegal PstI site found at 1975 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
Illegal PstI site found at 1002
Illegal PstI site found at 1975 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1002
Illegal PstI site found at 1975 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1002
Illegal PstI site found at 1975
Illegal NgoMIV site found at 737
Illegal NgoMIV site found at 764
Illegal NgoMIV site found at 2334 - 1000COMPATIBLE WITH RFC[1000]