Difference between revisions of "Part:BBa K2992017"
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===References=== | ===References=== | ||
| − | + | RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium. (2019). ACS Synthetic Biology, [online] p. Available at: https://pubs.acs.org/doi/abs/10.1021/acssynbio.9b00075 [Accessed 21 Oct. 2019]. | |
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| + | Minton, N., Ehsaan, M., Humphreys, C., Little, G., Baker, J., Henstra, A., Liew, F., Kelly, M., Sheng, L., Schwarz, K. and Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, pp.104-112. | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2992017 parameters</partinfo> | <partinfo>BBa_K2992017 parameters</partinfo> | ||
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Revision as of 13:31, 21 October 2019
5-UTR containing RBS for fdx gene from C. sporogenes
5’-UTR containing RBS for fdx gene from C. sporogenes
Usage and Biology
Ferredoxin is an iron-sulfur protein involved in central redox reactions occurring during the general metabolism of Clostridia. 5’-UTR and RBS region for fdx is found naturally downstream of Pfdx and upstream of the start codon. In our project we use the regulatory region for the fdx gene comprising Pfdx-t14c (BBa_K2992016) and this entry to regulate our volatile reporter genes for predicting the production of botulinum neurotoxin following food manufacture.
Characterisation
Data incoming
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium. (2019). ACS Synthetic Biology, [online] p. Available at: https://pubs.acs.org/doi/abs/10.1021/acssynbio.9b00075 [Accessed 21 Oct. 2019].
Minton, N., Ehsaan, M., Humphreys, C., Little, G., Baker, J., Henstra, A., Liew, F., Kelly, M., Sheng, L., Schwarz, K. and Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, pp.104-112.