Difference between revisions of "Part:BBa K2974310:Experience"
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− | The results for the toehold were confirmed through a dual plasmid transformation in BL21 <i>E. coli.</i> The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the toehold. | + | The results for the toehold were confirmed through a dual plasmid transformation in BL21 <i>E. coli.</i> The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the toehold. The dual plasmid transformation would not succeed without the correct <i>C. elegans</i> toehold intermediate, so the function of this part (BBa_K2974310) has been validated through experimentation and sequencing (see BBa_K2974316 for sequencing confirmation). |
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Revision as of 13:27, 21 October 2019
The results for the toehold were confirmed through a dual plasmid transformation in BL21 E. coli. The dual plasmid transformation presented greater fluorescence measured in lux values in comparison to the single switch transformation, confirming the function of the toehold. The dual plasmid transformation would not succeed without the correct C. elegans toehold intermediate, so the function of this part (BBa_K2974310) has been validated through experimentation and sequencing (see BBa_K2974316 for sequencing confirmation).
Applications of BBa_K2974310
This sequence is to be used as a proof of concept
User Reviews
UNIQ9c0b96d94d0c9e85-partinfo-00000000-QINU UNIQ9c0b96d94d0c9e85-partinfo-00000001-QINU