Difference between revisions of "Part:BBa K3257035"

Latest revision as of 13:20, 21 October 2019

Chloramphenicol Resistance Gene T33X (ChlR_T33X)

This part is a mutant of a gene encoding ChlR, a resistance protein of chloramphenicol, and it is nonsense mutated at its 33rd amino acid residual. We used this mutated ChlR as one of the target genes of our directed evolution process. After expression in BL21(DE3), we found that no colony grew on a medium plate containing chloramphenicol, showing its inability of being resistant to chloramphenicol.

Numbers of different types of colonies grown on plates with different antibiotics (Data of this part shown in red)

	No ChlR	WT ChlR	ChlR_T33X	ChlR_Y92X	ChlR_E97X	ChlR_S121X	ChlR_L158X	ChlR_K182X	ChlR_Q190X
Amp	19	192	291	128	236	138	255	175	276
Amp/Chl	0	30	0	0	0	0	0	0	0

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3257035 short</partinfo>
-This part is a mutant of a gene encoding ChlR, a resistance protein of chloramphenicol, and it is nonsense mutated at its 33rd amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we found that no colony grew on a medium plate containing chloramphenicol, showing its inability of being resistant to chloramphenicol.
+This part is a mutant of a gene encoding ChlR, a resistance protein of chloramphenicol, and it is nonsense mutated at its 33rd amino acid residual. We used this mutated ChlR as one of the target genes of our directed evolution process. After expression in BL21(DE3), we found that no colony grew on a medium plate containing chloramphenicol, showing its inability of being resistant to chloramphenicol.
 '''Numbers of different types of colonies grown on plates with different antibiotics (Data of this part shown in red)'''