Difference between revisions of "Part:BBa K3093007"

Revision as of 13:19, 21 October 2019

His tag-cex-hlyA

Usage and Biology

Cellulomonas fimi exo-beta-1,4-glucanase and beta-1,4-xylanase gene（cex) with a his tag and a hlyA tag.

In Cellulomonas fimi the cex gene encodes an exoglucanase (Exg) involved in the degradation of cellulose.The codon usage in C. fimi appears to be quite different from that of Escherichia coli. A dramatic (98.5%) bias occurs for G or C in the third position for the 35 codons utilized in the cex gene. For more info visit https://www.ncbi.nlm.nih.gov/nuccore/L11080.1

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 578
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 36
Illegal XhoI site found at 45
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 211
Illegal NgoMIV site found at 584
Illegal NgoMIV site found at 1086
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 631
Illegal SapI.rc site found at 714

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3093007 short</partinfo>
-Cellulomonas fimi exo-beta-1,4-glucanase and beta-1,4-xylanase（cex)
 ===Usage and Biology===
+Cellulomonas fimi exo-beta-1,4-glucanase and beta-1,4-xylanase gene（cex) with a his tag and a hlyA tag.
 In Cellulomonas fimi the cex gene encodes an exoglucanase (Exg) involved in the degradation of cellulose.The codon usage in C. fimi appears to be quite different from that of Escherichia coli. A dramatic (98.5%) bias occurs for G or C in the third position for the 35 codons utilized in the cex gene.
 For more info visit https://www.ncbi.nlm.nih.gov/nuccore/L11080.1