Difference between revisions of "Part:BBa K3113005"

Revision as of 13:09, 21 October 2019

FLuc

This DNA sequence codes for the firefly luciferase. The firefly luciferase (FLuc) is a light-emitting enzyme. This sequence has its origin in the organism Photinus pyralis.

Usage

The readout of ALiVE is based on RNA. We used FLuc as message loaded into the vesicles. This had the advantage, that we could test our vesicles for passive transfection. The cells treated with our vesicle could be examined for passive transfection via a FLuc assay.

Biology

FLuc is an oxidative enzyme, producing bioluminescence in the presence of the substrate luciferin and oxygen.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 This sequence has its origin in the organism Photinus pyralis.
+<h2>Usage</h2>
-<!-- Add more about the biology of this part here
+The readout of ALiVE is based on RNA. We used FLuc as message loaded into the vesicles. This had the advantage, that we could test our vesicles for passive transfection. The cells treated with our vesicle could be examined for passive transfection via a FLuc assay.
-===Usage and Biology===
+<h2>Biology</h2>
+FLuc is an oxidative enzyme, producing bioluminescence in the presence of the substrate luciferin and oxygen.
+<h2>Characterization</h2>
 <!-- -->
@@ Line 14: / Line 20: @@
 <partinfo>BBa_K3113005 SequenceAndFeatures</partinfo>
+<h2>Usage</h2>
 <!-- Uncomment this to enable Functional Parameter display