Difference between revisions of "Part:BBa K3081057"
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This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the bottom strand of initiation site on p15A origin. In this part, we add a degradation signal peptide "ssrA" to the dCas9 to alleviate its effect.  
 
This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the bottom strand of initiation site on p15A origin. In this part, we add a degradation signal peptide "ssrA" to the dCas9 to alleviate its effect.  
 
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https://2019.igem.org/wiki/images/3/3b/T--Peking--p15A_control_schematic.png
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https://2019.igem.org/File:T--Peking--p15A_mechanism.png
 
</center>
 
</center>
 
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Revision as of 13:00, 21 October 2019

CRISPR-based replication interference system for p15A plasmid copy number control, ini(+) site

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the bottom strand of initiation site on p15A origin. In this part, we add a degradation signal peptide "ssrA" to the dCas9 to alleviate its effect.

https://2019.igem.org/File:T--Peking--p15A_mechanism.png

Mechanism of CRISPRri-based p15A plasmid copy number control

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 2321
    Illegal NheI site found at 5495
    Illegal NheI site found at 5518
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961