Difference between revisions of "Part:BBa K2974400:Design"
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
By using the lin-4 gene pre-mRNA primary transcript F59G1.6 sequence found on WormBank, we obtained a complementary RNA trigger sequence. By inputting the minimum free energy structures for each of the switch and trigger combinations, NUPACK showed the possible structure, confirming that our selected trigger and toehold combination has a high probability of working. | By using the lin-4 gene pre-mRNA primary transcript F59G1.6 sequence found on WormBank, we obtained a complementary RNA trigger sequence. By inputting the minimum free energy structures for each of the switch and trigger combinations, NUPACK showed the possible structure, confirming that our selected trigger and toehold combination has a high probability of working. | ||
| + | <br> | ||
<br> | <br> | ||
<center> | <center> | ||
Latest revision as of 12:56, 21 October 2019
T7 C. elegans Trigger
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 159
Illegal NotI site found at 6
Illegal NotI site found at 153 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 69
Design Notes
By using the lin-4 gene pre-mRNA primary transcript F59G1.6 sequence found on WormBank, we obtained a complementary RNA trigger sequence. By inputting the minimum free energy structures for each of the switch and trigger combinations, NUPACK showed the possible structure, confirming that our selected trigger and toehold combination has a high probability of working.
Source
The lin-4 gene pre-mRNA primary transcript F59G1.6 (non-toxic) is native to the genome of C. elegans.