Difference between revisions of "Part:BBa K3113008"
Theresakeil (Talk | contribs) |
Theresakeil (Talk | contribs) |
||
| Line 8: | Line 8: | ||
<h2>Usage</h2> | <h2>Usage</h2> | ||
| − | + | HiBiT allows you to quantify the amount of tagged protein relatively quick via a luminescence assay. We are using this part fused to the vesicle forming proteins to measure the export rate of vesicles and the efficiency of purification. | |
<h2>Biology</h2> | <h2>Biology</h2> | ||
Revision as of 12:46, 21 October 2019
HiBiT
HiBiT is a peptide tag used for antibody-free endogenous protein detection. It is 11 amino acids long and binds with high affinity to another larger subunit called LgBiT. The bound complex has luciferase activity. Promega has all rights to this sequence.
Usage
HiBiT allows you to quantify the amount of tagged protein relatively quick via a luminescence assay. We are using this part fused to the vesicle forming proteins to measure the export rate of vesicles and the efficiency of purification.
Biology
“The Nano-Glo® HiBiT Lytic Detection System quantifies cellular protein in minutes with high sensitivity and a broad dynamic range using a simple add-mix-read assay format.”[1] The HiBiT peptide is an 11 amino acid long tag that can be added to the protein of interest. The tagged protein can be measured with the Nano-Glo® HiBiT Detection Reagent which contains the LbBiT. Together HiBiT and LgBiT form a functioning luminescent NanoBiT® enzyme.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]