Difference between revisions of "Part:BBa K3088001"

Revision as of 12:42, 21 October 2019

HBEGF/ENVZ Chimeric Complete

Heparin-binding EGF-like growth factor (HB-EGF) is a unique receptor of the diphtheria toxin. EnvZ, on the other hand, is a member of two-component regulatory system that is important for bacteria osmoregulation. When diphteria toxin binds to HB-EGF, it will phosphorylate EnvZ and activates the signalling cascade pathway. Once EnvZ is phosphorylated, OmpR (a transcription factor of OmpC promoter) will also be phosphorylated. The phosphorylated OmpR will initiate OmpC transcription and express the gene following OmpC promoter. This is the complete part containing the appropriate promoter T5 lac promoter (BBa_K592008), Ribosomal Binding Site (BBa_B0034), our HBEGF/EnvZ chimeric protein (BBa_K3088001), Histidine tag (BBa_K1223006), and double terminator (BBa_B0010 and BBa_B0012)

Usage and Biology

This part when combined with OMPC/GFP part (BBa_K769001) can be used for diphteria toxin detection with observable green fluorescent protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 12:41, 21 October 2019 (view source) Enga2 (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Revision as of 12:42, 21 October 2019 (view source) Enga2 (Talk \| contribs) (→‎Usage and Biology) Newer edit →
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	This part when combined with OMPC/GFP part (BBa_K769001) can be used for diphteria toxin detection with observable green fluorescent protein.		This part when combined with OMPC/GFP part (BBa_K769001) can be used for diphteria toxin detection with observable green fluorescent protein.

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−	<table style="border-collapse:collapse;border-spacing:0;border-color:#ccc" class="tg"><tr><th style="font-family:Arial, sans-serif;font-size:14px;font-weight:bold;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;text-align:center;vertical-align:middle" rowspan="2">Treatment</th><th style="font-family:Arial, sans-serif;font-size:14px;font-weight:bold;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;text-align:center;vertical-align:top" colspan="11">Duplicate</th><th style="font-family:Arial, sans-serif;font-size:14px;font-weight:bold;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;text-align:center;vertical-align:middle" rowspan="2">Average</th></tr><tr><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">1</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">2</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">3</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">4</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">5</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">6</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">7</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">8</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">9</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">10</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">11</td></tr><tr><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 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5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">154135753</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">149680163</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">150141938</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 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5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">156934751</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">152951136</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">157435211</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">164726673</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">164550358</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">164104823</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#f9f9f9;text-align:center;vertical-align:top">158001381</td></tr></table>