Difference between revisions of "Part:BBa K3088001"

Revision as of 12:41, 21 October 2019

HBEGF/ENVZ Chimeric Complete

Heparin-binding EGF-like growth factor (HB-EGF) is a unique receptor of the diphtheria toxin. EnvZ, on the other hand, is a member of two-component regulatory system that is important for bacteria osmoregulation. When diphteria toxin binds to HB-EGF, it will phosphorylate EnvZ and activates the signalling cascade pathway. Once EnvZ is phosphorylated, OmpR (a transcription factor of OmpC promoter) will also be phosphorylated. The phosphorylated OmpR will initiate OmpC transcription and express the gene following OmpC promoter. This is the complete part containing the appropriate promoter T5 lac promoter (BBa_K592008), Ribosomal Binding Site (BBa_B0034), our HBEGF/EnvZ chimeric protein (BBa_K3088001), Histidine tag (BBa_K1223006), and double terminator (BBa_B0010 and BBa_B0012)

Usage and Biology

This part when combined with OMPC/GFP part (BBa_K769001) can be used for diphteria toxin detection with observable green fluorescent protein.

Treatment	Duplicate											Average
Treatment	1	2	3	4	5	6	7	8	9	10	11	Average
A	159135151	170652003	166127901	162815457	164648665	169695170	167532349	164196084	166116493	166599137	166832993	165850128
B	157298655	166717172	167267390	162280640	167369857	163968636	165108326	165459144	163678613	167407871	162760768	164483370
C	154135753	149680163	150141938	158315713	147496405	158925251	156372992	157634375	159631216	158575707	160202437	155555632
D	158499813	155810193	153257096	155180693	154564445	156934751	152951136	157435211	164726673	164550358	164104823	158001381

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 15:24, 20 October 2019 (view source) Enga2 (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Revision as of 12:41, 21 October 2019 (view source) Enga2 (Talk \| contribs) (→‎Usage and Biology) Newer edit →
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	This part when combined with OMPC/GFP part (BBa_K769001) can be used for diphteria toxin detection with observable green fluorescent protein.		This part when combined with OMPC/GFP part (BBa_K769001) can be used for diphteria toxin detection with observable green fluorescent protein.

		+
		+	<table style="border-collapse:collapse;border-spacing:0;border-color:#ccc" class="tg"><tr><th style="font-family:Arial, sans-serif;font-size:14px;font-weight:bold;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;text-align:center;vertical-align:middle" rowspan="2">Treatment</th><th style="font-family:Arial, sans-serif;font-size:14px;font-weight:bold;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;text-align:center;vertical-align:top" colspan="11">Duplicate</th><th style="font-family:Arial, sans-serif;font-size:14px;font-weight:bold;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;text-align:center;vertical-align:middle" rowspan="2">Average</th></tr><tr><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">1</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">2</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">3</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">4</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">5</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">6</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">7</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">8</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">9</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">10</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#efefef;font-weight:bold;text-align:center;vertical-align:top">11</td></tr><tr><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;font-weight:bold;text-align:center;vertical-align:top">A</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">159135151</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">170652003</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">166127901</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:center;vertical-align:top">162815457</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">164648665</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">169695170</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">167532349</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">164196084</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">166116493</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:center;vertical-align:top">166599137</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">166832993</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#fff;text-align:center;vertical-align:top">165850128</td></tr><tr><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;font-weight:bold;text-align:center;vertical-align:top">B</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">157298655</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">166717172</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">167267390</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">162280640</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">167369857</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">163968636</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">165108326</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:center;vertical-align:top">165459144</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:center;vertical-align:top">163678613</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">167407871</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">162760768</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#f9f9f9;text-align:center;vertical-align:top">164483370</td></tr><tr><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;font-weight:bold;text-align:center;vertical-align:top">C</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">154135753</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">149680163</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">150141938</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">158315713</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">147496405</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:center;vertical-align:top">158925251</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">156372992</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">157634375</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">159631216</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">158575707</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">160202437</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#fff;text-align:center;vertical-align:top">155555632</td></tr><tr><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;font-weight:bold;text-align:center;vertical-align:top">D</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">158499813</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">155810193</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">153257096</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">155180693</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">154564445</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">156934751</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">152951136</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">157435211</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">164726673</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">164550358</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#ffffff;text-align:left;vertical-align:top">164104823</td><td style="font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:0px;overflow:hidden;word-break:normal;border-top-width:1px;border-bottom-width:1px;border-color:#000000;color:#333;background-color:#f9f9f9;text-align:center;vertical-align:top">158001381</td></tr></table>