Difference between revisions of "Part:BBa E0022"
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In ECUST_China 2019 characterization，we constructed a plasmid containing pLac and CI protein, adding BBa_E0022 eCFP as the reporter gene. We attempted to verify the function of this plasmid via observing the eCFP fluorescence by the comparison between the negative control and induced cells. | In ECUST_China 2019 characterization，we constructed a plasmid containing pLac and CI protein, adding BBa_E0022 eCFP as the reporter gene. We attempted to verify the function of this plasmid via observing the eCFP fluorescence by the comparison between the negative control and induced cells. | ||
| − | <html><div class="exper-com-box"><img style="width:500px;" src="https://static.igem.org/mediawiki/parts/7/7f/T--ECUST_China--Figure.1_Fluorescence_intensity_of_eCFP.jpg"> <br><span style="font-size: 14px;"> | + | |
| + | <html><style> | ||
| + | .exper-com-box{ | ||
| + | width: 80%; | ||
| + | text-align: center; | ||
| + | padding-bottom: 20px; | ||
| + | }</style> | ||
| + | <div class="exper-com-box"><img style="width:500px;" src="https://static.igem.org/mediawiki/parts/7/7f/T--ECUST_China--Figure.1_Fluorescence_intensity_of_eCFP.jpg"> <br><span style="font-size: 14px;"> | ||
<b>Figure 1.</b> Fluorescence intensity of eCFP</span></div> </html> | <b>Figure 1.</b> Fluorescence intensity of eCFP</span></div> </html> | ||
However, since the CI and eCFP was polycistron, both under the control of pLac, the expression of eCFP was disturbed by CI. The figure illustrated that as time went by, the expression of eCFP decreased, contrary to the prospective results. The characterization of eCFP in ECUST_China showed that BBa_E0022 was not suitable for the characterization of polycistron structure. | However, since the CI and eCFP was polycistron, both under the control of pLac, the expression of eCFP was disturbed by CI. The figure illustrated that as time went by, the expression of eCFP decreased, contrary to the prospective results. The characterization of eCFP in ECUST_China showed that BBa_E0022 was not suitable for the characterization of polycistron structure. | ||
Revision as of 12:26, 21 October 2019
enhanced cyan fluorescent protein derived from A. victoria GFP
Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
ECUST_China 2019 Charaterization
Characterization
In ECUST_China 2019 characterization，we constructed a plasmid containing pLac and CI protein, adding BBa_E0022 eCFP as the reporter gene. We attempted to verify the function of this plasmid via observing the eCFP fluorescence by the comparison between the negative control and induced cells.
Figure 1. Fluorescence intensity of eCFP
However, since the CI and eCFP was polycistron, both under the control of pLac, the expression of eCFP was disturbed by CI. The figure illustrated that as time went by, the expression of eCFP decreased, contrary to the prospective results. The characterization of eCFP in ECUST_China showed that BBa_E0022 was not suitable for the characterization of polycistron structure.