Difference between revisions of "Part:BBa K108029:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K108029 short</partinfo>
 
<partinfo>BBa_K108029 short</partinfo>
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===Source===
 
===Source===
  
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pWW0
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XylS was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).
  
 
===References===
 
===References===
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1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373.
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 +
2. NCBI.

Latest revision as of 08:48, 25 October 2008

XylS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 754
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 217


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

XylS was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).

References

1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373.

2. NCBI.