Difference between revisions of "Part:BBa K2411007"
Line 6: Line 6:
  
 
Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.<br>
 
Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.<br>
 +
 
<h2><strong>Characterization from SCUT_China 2019:</strong></h2>
 
<h2><strong>Characterization from SCUT_China 2019:</strong></h2>
−
In SCUT_China 2019's project, wthis Toehold Switch also named toehold switch B.<br>
+
In SCUT_China 2019's project, Toehold Switch 2 also named toehold switch B.<br>
−
The new classes of regulatory components offer wide dynamic range and low system crosstalk. SCUT_China 2019 selected four toehold switches, including Toehold Switch B, with widest dynamic range and highest orthogonality used for multiplexing systems and provided by the reference, which are switch A&B&C&D and trigger A&B&C&D, to regulate the expressions of four acid-resistance factors.
+
The new classes of regulatory components offer wide dynamic range and low system crosstalk. SCUT_China 2019 selected four toehold switches, including Toehold Switch B, with widest dynamic range and highest orthogonality used for multiplexing systems and provided by the reference, which are switch A&B&C&D and trigger A&B&C&D, to regulate the expressions of four acid-resistance factors.<br>
 +
 
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:13, 21 October 2019


Toehold 2

This sequence is the second best performing forward engineered toehold from Green et. al. A toehold is an RNA based riboregulator that, in the presence of a trigger RNA, allows for the expression of a downstream gene. This part contains the hairpin loop sequence with an RBS. There is no gene associated with this toehold.

Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.

Characterization from SCUT_China 2019:

In SCUT_China 2019's project, Toehold Switch 2 also named toehold switch B.
The new classes of regulatory components offer wide dynamic range and low system crosstalk. SCUT_China 2019 selected four toehold switches, including Toehold Switch B, with widest dynamic range and highest orthogonality used for multiplexing systems and provided by the reference, which are switch A&B&C&D and trigger A&B&C&D, to regulate the expressions of four acid-resistance factors.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]