Difference between revisions of "Part:BBa K108028:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K108028 short</partinfo>
 
<partinfo>BBa_K108028 short</partinfo>
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===Source===
 
===Source===
  
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pWW0
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XylB was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).
  
 
===References===
 
===References===
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1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373.
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 +
2. NCBI.

Latest revision as of 08:42, 25 October 2008

XylB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 296


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

XylB was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).

References

1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373.

2. NCBI.