Difference between revisions of "Part:BBa K2411004"

Revision as of 12:13, 21 October 2019

Forward Engineered Toehold 1

This sequence is the forward engineered toehold from Green et. al. A toehold is an RNA based riboregulator that, in the presence of a trigger RNA, allows for the expression of a downstream gene. This part contains the hairpin loop sequence that with the RBS. There is no gene associated with this toehold.

Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.

Characterization from SCUT_China 2019:

In SCUT_China 2019's project, Toehold Switch 1 also named toehold switch D.
The new classes of regulatory components offer wide dynamic range and low system crosstalk. SCUT_China 2019 selected four toehold switches, including Toehold Switch D, with widest dynamic range and highest orthogonality used for multiplexing systems and provided by the reference, which are switch A&B&C&D and trigger A&B&C&D, to regulate the expressions of four acid-resistance factors.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 In SCUT_China 2019's project,  Toehold Switch 1 also named toehold switch D.<br>
 The new classes of regulatory components offer wide dynamic range and low system crosstalk. SCUT_China 2019 selected four toehold switches, including Toehold Switch D, with widest dynamic range and highest orthogonality used for multiplexing systems and provided by the reference, which are switch A&B&C&D and trigger A&B&C&D, to regulate the expressions of four acid-resistance factors.<br>
-<h2><strong>Characterization:</strong></h2>