Difference between revisions of "Part:BBa K108027:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K108027 short</partinfo>
 
<partinfo>BBa_K108027 short</partinfo>
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===Source===
 
===Source===
  
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pWW0
+
XylM was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).
  
 
===References===
 
===References===
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1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373.
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 +
2. NCBI.

Latest revision as of 08:37, 25 October 2008

XylM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

XylM was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).

References

1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341-373.

2. NCBI.