Difference between revisions of "Part:BBa K2926052"
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<img class="figure image" src="https://2019.igem.org/wiki/images/3/31/T--Bielefeld-CeBiTec--Fluorescence_norm..png"> | <img class="figure image" src="https://2019.igem.org/wiki/images/3/31/T--Bielefeld-CeBiTec--Fluorescence_norm..png"> | ||
| − | <figcaption> | + | <figcaption> <b>Fig. 1: Emission spectrum of our mCherry-marked Toygenics with mCherry as a comparison measured with TECAN infinite M200 plate reader(λ[Ex]=570 nm, λ[Em]=600 nm to 850 nm).</b></figcaption> |
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Latest revision as of 12:08, 21 October 2019
Fusion protein of mCherry and M13 bacteriophage protein pVIII
The major coat protein pVIII of the M13 bacteriophage was fused c-terminally to the fluorescence reporter protein mCherry.
Usage and Biology
This part was used during Troygenics Assembly to visualize the produced M13 bacteriophage-derived Troygenics via fluorescence measurement (Fig. 1).
We compared the emission spectrum of our Troygenics with the emission spectrum of mCherry, because the fluorescence marker cloned on the Troygenics is mCherry, too. We have seen a difference in the emission peak of about 20 nm. This is probably because the mCherry on our Troygenics is fused to the whole Troygenic. Because of this, there might be a different folding resulting in a shift of the emission spectrum.
Sequence and Features
Sequence was validated by Sanger sequencing.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]