Difference between revisions of "Part:BBa K2992000"
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===Characterisation=== | ===Characterisation=== | ||
| − | + | This part was used as part of our FAST reporter constructs - [https://parts.igem.org/Part:BBa_K2992042 BBa_K2992042],[https://parts.igem.org/Part:BBa_K2992043 BBa_K2992043],[https://parts.igem.org/Part:BBa_K2992044 BBa_K2992044],[https://parts.igem.org/Part:BBa_K2992045 BBa_K2992045], | |
| + | [https://parts.igem.org/Part:BBa_K2992046 BBa_K2992046], [https://parts.igem.org/Part:BBa_K2992047 BBa_K2992047],[https://parts.igem.org/Part:BBa_K2992048 BBa_K2992048] - which were characterised using the FAST fluorometric assay.... <br><br><br> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
Revision as of 12:08, 21 October 2019
FAST reporter gene
Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST) reporter gene.
Usage and Biology
FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from Halorhodospira halophila and has been codon optimised for fluorescence studies in the genus Clostridium. In our project we use FAST as a reporter to demonstrate the activity of our chosen promoters and to assess its suitability as an alternative non-volatile reporter system for predicting neurotoxin production.
Characterisation
This part was used as part of our FAST reporter constructs - BBa_K2992042,BBa_K2992043,BBa_K2992044,BBa_K2992045,
BBa_K2992046, BBa_K2992047,BBa_K2992048 - which were characterised using the FAST fluorometric assay....
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 155
References
Street et al., 2019 to be updated