Difference between revisions of "Part:BBa K108025:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K108025 short</partinfo>
 
<partinfo>BBa_K108025 short</partinfo>
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===Source===
 
===Source===
  
−
pWW0
+
Pu was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).
  
 
===References===
 
===References===
 +
1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341.
 +
 +
2. NCBI.

Revision as of 08:26, 25 October 2008

Pu


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 163
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

Pu was cloned from pWW0 of Pseudomonas putida (reference 1 and 2).

References

1. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annual Review of Microbiology. 1997, 51:341.

2. NCBI.