Difference between revisions of "Part:BBa K3098009"

 
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<partinfo>BBa_K3098009 short</partinfo>
 
<partinfo>BBa_K3098009 short</partinfo>
  
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This part is the one we are hoping to use in the production of the functional PgD5. In this part, GST tag helps the dissolution and purification of the peptide while Avitag provides a site for biotinylation as a linkage to the dCBM3a and BC.
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This part is the one we are hoping to use in the production of the functional PgD5. In this part, GST tag helps the dissolution and purification of the peptide while Avitag provides a site for biotinylation as a linkage to the dCBM3a and BC. It has been proved by our experiments that these modifications on the peptide will not eliminate the antifungal function of the peptides after our corresponding treatment, so we'll be able to produce functional antimicrobial peptides with this precursor.
  
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===Usage and Biology===
 
  
 
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Revision as of 12:01, 21 October 2019


GST+PgD5+Avitag

This part is the one we are hoping to use in the production of the functional PgD5. In this part, GST tag helps the dissolution and purification of the peptide while Avitag provides a site for biotinylation as a linkage to the dCBM3a and BC. It has been proved by our experiments that these modifications on the peptide will not eliminate the antifungal function of the peptides after our corresponding treatment, so we'll be able to produce functional antimicrobial peptides with this precursor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85