Difference between revisions of "Part:BBa K3021005"

 
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<partinfo>BBa_K3021005 parameters</partinfo>
 
<partinfo>BBa_K3021005 parameters</partinfo>
 
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<h2>Sequencing and sequence alignment of K3021005</h2>
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< img src="https://2019.igem.org/wiki/images/5/56/T--PuiChing_Macau--Alignment_1.jpg">
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<div class="legend">Figure 3. Representative sequencing results of K3021005_pSCBe (pilA_K863030_pSCBe)
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<p>To future confirm that our constructs have correct sequence, we send our clones (with positive PCR results) out to sequencing.</p >
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<p>The sequencing results show that we have successfully assembled target genes K3021005 (pilA_K863030_6xHis) to the vector (pSCBe).
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Revision as of 11:53, 21 October 2019


pilA-Laccase-HisTag

This is a new composite part designed to allow Laccase secretion from our engineered cyanobacteria through embedded the secretion peptide, pilA1. Laccase is an important enzyme, which would degrade different chemicals. The Laccase sequence in this part is based on the laccase cDNA sequence from Trametes versicolor in iGEM part k863030. Secretion of heterologous proteins into cell culture medium offers significant advantages for downstream processing over production. pilA1 is the commonly know with its effect on the cyanobacteria secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 928
    Illegal BglII site found at 1360
    Illegal XhoI site found at 960
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1599
    Illegal AgeI site found at 327
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal SapI.rc site found at 392







Sequencing and sequence alignment of K3021005


< img src="https://2019.igem.org/wiki/images/5/56/T--PuiChing_Macau--Alignment_1.jpg">


Figure 3. Representative sequencing results of K3021005_pSCBe (pilA_K863030_pSCBe)


To future confirm that our constructs have correct sequence, we send our clones (with positive PCR results) out to sequencing.



The sequencing results show that we have successfully assembled target genes K3021005 (pilA_K863030_6xHis) to the vector (pSCBe).