Difference between revisions of "Part:BBa K108019:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K108019 short</partinfo>
 
<partinfo>BBa_K108019 short</partinfo>
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===Source===
 
===Source===
  
−
pBHR68
+
phbC was cloned from pBHR68 (reference 1).
  
 
===References===
 
===References===
 +
1. Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(beta-Hydroxybutyrate) Production. Journal of Bioscience and Bioengineering. 2000, 89(4):307-311.

Latest revision as of 07:28, 25 October 2008

PhbC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 192
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 541
    Illegal NgoMIV site found at 853
    Illegal NgoMIV site found at 1132
    Illegal NgoMIV site found at 1545
    Illegal NgoMIV site found at 1612
    Illegal AgeI site found at 280
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR using primers with biobrick prefix and surfix, cloned into pSB1AC3, and then eliminate Pst1 and Not1 using site-directed mutation.


Source

phbC was cloned from pBHR68 (reference 1).

References

1. Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(beta-Hydroxybutyrate) Production. Journal of Bioscience and Bioengineering. 2000, 89(4):307-311.