Difference between revisions of "Part:BBa K3098001"
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GxCesA protein is a GT-2 glycosyltransferase containing transmembrane helices and the catalytic subunit of cellulose synthase. | GxCesA protein is a GT-2 glycosyltransferase containing transmembrane helices and the catalytic subunit of cellulose synthase. | ||
− | The first cellulose synthase gene was identified in ''Gluconacetobacter xylinus'' (formly ''Acetobacter xylinum''). In this bacterium, four genes are clustered in one operon, namely, ''gxcesA'', ''gxcesB'', ''gxcesC'' and ''gxcesD'' (formly ''bcsA'', ''bcsB'', ''bcsC'' and ''bcsD''). The gene of cesA is found in all living cellulose-producing organisms, whereas the other genes are specific to bacterial cellulose synthase. | + | The first cellulose synthase gene was identified in ''Gluconacetobacter xylinus'' (formly ''Acetobacter xylinum''). In this bacterium, four genes are clustered in one operon, namely, ''gxcesA'', ''gxcesB'', ''gxcesC'' and ''gxcesD'' (formly ''bcsA'', ''bcsB'', ''bcsC'' and ''bcsD''). The gene of ''cesA'' is found in all living cellulose-producing organisms, whereas the other genes are specific to bacterial cellulose synthase. |
In the case of bacteria, the CesA and CesB proteins represent the minimum subunit requirements for cellulose synthesis. | In the case of bacteria, the CesA and CesB proteins represent the minimum subunit requirements for cellulose synthesis. | ||
− | '''This part cannot work | + | '''This part cannot work alone. See Cellulose Synthase''' ([[Part:BBa_K3098005]]). |
− | + | ---- | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
<!-- --> | <!-- --> | ||
− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'>'''Sequence and Features'''</span> |
<partinfo>BBa_K3098001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3098001 SequenceAndFeatures</partinfo> | ||
Latest revision as of 11:48, 21 October 2019
GxCesA
GxCesA protein is a GT-2 glycosyltransferase containing transmembrane helices and the catalytic subunit of cellulose synthase. The first cellulose synthase gene was identified in Gluconacetobacter xylinus (formly Acetobacter xylinum). In this bacterium, four genes are clustered in one operon, namely, gxcesA, gxcesB, gxcesC and gxcesD (formly bcsA, bcsB, bcsC and bcsD). The gene of cesA is found in all living cellulose-producing organisms, whereas the other genes are specific to bacterial cellulose synthase. In the case of bacteria, the CesA and CesB proteins represent the minimum subunit requirements for cellulose synthesis.
This part cannot work alone. See Cellulose Synthase (Part:BBa_K3098005).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 580
Illegal PstI site found at 742 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 580
Illegal PstI site found at 742 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 580
Illegal BglII site found at 1142
Illegal XhoI site found at 232 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 580
Illegal PstI site found at 742 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 580
Illegal PstI site found at 742
Illegal NgoMIV site found at 212
Illegal AgeI site found at 2193 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1717