Difference between revisions of "Part:BBa K2924054:Design"
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===Source===
 
===Source===
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PBSMul1 plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of a-s2-casein. a-s2-casein was commercial synthesized by GenScript.
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For sources see the individual sources of the parts.
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Revision as of 11:47, 21 October 2019


Hpall + SPYurl + alpha s2 + fd terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 557

Design Notes

Expression system for the expression and secretion of the protein in B. subtilis

Source

PBSMul1 plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of a-s2-casein. a-s2-casein was commercial synthesized by GenScript.