Difference between revisions of "Part:BBa K108018:Design"
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===References===
 
===References===
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1. Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(beta-Hydroxybutyrate) Production. Journal of Bioscienve and Bioengineering. 2000, 89(4):307-311.
+
1. Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(beta-Hydroxybutyrate) Production. Journal of Bioscience and Bioengineering. 2000, 89(4):307-311.

Revision as of 07:07, 25 October 2008

PhbAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 121
    Illegal NgoMIV site found at 217
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 237
    Illegal NgoMIV site found at 334
    Illegal NgoMIV site found at 574
    Illegal NgoMIV site found at 715
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1796


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

phbAB was cloned from pBHR68 (reference 1).

References

1. Construction and Selection of the Novel Recombinant Escherichia coli Strain for Poly(beta-Hydroxybutyrate) Production. Journal of Bioscience and Bioengineering. 2000, 89(4):307-311.