Difference between revisions of "Part:BBa K2924033:Design"

(→‎Design Notes)
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===References===
 
===References===
 +
Brockmeier, U., et al. (2006). "Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria." J Mol Biol 362(3): 393-402.
 +
 +
Knapp, A., et al. (2017). "Activity-independent screening of secreted proteins using split GFP." J Biotechnol 258: 110-116.

Revision as of 11:43, 21 October 2019


Hpall + SPNprE + α-s1-casein + fd terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Expression system for the expression and secretion of the protein in B. subtilis

Source

PBSMul1 plasmid was kindly provided by Dr. Andreas Knapp from the research center Jülich. The DNA sequence was acquired by reverse-translating the protein sequence of a-s1-casein. a-s1-casein was commercial synthesized by GenScript.

References

Brockmeier, U., et al. (2006). "Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria." J Mol Biol 362(3): 393-402.

Knapp, A., et al. (2017). "Activity-independent screening of secreted proteins using split GFP." J Biotechnol 258: 110-116.