Difference between revisions of "Part:BBa K3007001:Design"
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[[File:T--QHFZ-China--BBa K3007001 1.png|400px|thumb|left|Figure 1. Schematic cartoon of the DNA construct of BBa_K3007001]] | [[File:T--QHFZ-China--BBa K3007001 1.png|400px|thumb|left|Figure 1. Schematic cartoon of the DNA construct of BBa_K3007001]] | ||
When we used the part, the sequence at the 5' end of this part in the lac promoter (ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa) was retained. | When we used the part, the sequence at the 5' end of this part in the lac promoter (ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa) was retained. | ||
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It is a promoter modified from lac promoter. As at the 3' end of lac promoter, there is a RBS sequence RBS0029 (tcacacaggaaa). As a result, no additional RBS is needed. | It is a promoter modified from lac promoter. As at the 3' end of lac promoter, there is a RBS sequence RBS0029 (tcacacaggaaa). As a result, no additional RBS is needed. | ||
Latest revision as of 11:43, 21 October 2019
Uric acid-responsive promoter (PhucR)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When we used the part, the sequence at the 5' end of this part in the lac promoter (ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa) was retained.
It is a promoter modified from lac promoter. As at the 3' end of lac promoter, there is a RBS sequence RBS0029 (tcacacaggaaa). As a result, no additional RBS is needed.
Source
The key component hucO comes from Deinococcus radiodurans.