Difference between revisions of "Part:BBa K2926086"
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| − | This part enables the expression of Cas13a (Lwa) and a guide RNA array containing three guide RNAs that target essential genes in S. cerevisiae. | + | This part enables the expression of Cas13a (Lwa) and a guide RNA array containing three guide RNAs that target essential genes in <i>S. cerevisiae</i>. |
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Latest revision as of 11:43, 21 October 2019
Basic Insert to express Cas13a (Lwa) and a short guideRNA array in S. cerevisiae
This part enables the expression of Cas13a (Lwa) and a guide RNA array containing three guide RNAs that target essential genes in S. cerevisiae.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4694
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4719
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1730
Illegal BglII site found at 3410 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4694
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4694
Illegal AgeI site found at 55 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2809