Difference between revisions of "Part:BBa K3007001:Design"

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===Design Notes===
 
===Design Notes===
 
[[File:T--QHFZ-China--BBa K3007001 1.png|400px|thumb|left|Figure 1. Schematic cartoon of the DNA construct of BBa_K3007001]]
 
[[File:T--QHFZ-China--BBa K3007001 1.png|400px|thumb|left|Figure 1. Schematic cartoon of the DNA construct of BBa_K3007001]]
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When we used the part, the sequence at the 5' end of this part in the Lactose operon promoter (ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa) was retained.
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When we used the part, the sequence at the 5' end of this part in the lac promoter (ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa) was retained.
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<br>
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It is a promoter modified from lac promoter. As at the 3' end of lac promoter, there is a RBS sequence RBS0029 (tcacacaggaaa). As a result, no additional RBS is needed.
  
 
===Source===
 
===Source===

Revision as of 11:43, 21 October 2019


Uric acid-responsive promoter (PhucR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Figure 1. Schematic cartoon of the DNA construct of BBa_K3007001

When we used the part, the sequence at the 5' end of this part in the lac promoter (ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaa) was retained.
It is a promoter modified from lac promoter. As at the 3' end of lac promoter, there is a RBS sequence RBS0029 (tcacacaggaaa). As a result, no additional RBS is needed.

Source

The key component hucO comes from Deinococcus radiodurans.

References