Difference between revisions of "Part:BBa K2926085"
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− | This part enables the expression of Cas13a (Lsh) and a guide RNA array containing seven guide RNAs that target essential genes in S. cerevisiae. | + | This part enables the expression of Cas13a (Lsh) and a guide RNA array containing seven guide RNAs that target essential genes in <i>S. cerevisiae</i>. |
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Latest revision as of 11:39, 21 October 2019
Basic Insert to express Cas13a (Lsh) and a guideRNA array in S. cerevisiae
This part enables the expression of Cas13a (Lsh) and a guide RNA array containing seven guide RNAs that target essential genes in S. cerevisiae.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 5227
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 5252
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1016
Illegal BglII site found at 1562
Illegal BglII site found at 1640
Illegal BglII site found at 2327
Illegal BglII site found at 3215
Illegal BglII site found at 4205 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 5227
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 5227
Illegal AgeI site found at 55 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 783
Illegal SapI.rc site found at 1108
Illegal SapI.rc site found at 1978
Illegal SapI.rc site found at 2812