Difference between revisions of "Part:BBa K2926084"

 
 
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<partinfo>BBa_K2926084 short</partinfo>
 
<partinfo>BBa_K2926084 short</partinfo>
  
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This part enables the expression of Cas13a (Lbu) and a guide RNA array containing seven guide RNAs that target essential genes in S. cerevisiae
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This part enables the expression of Cas13a (Lbu) and a guide RNA array containing seven guide RNAs that target essential genes in <i>S. cerevisiae</i>.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:37, 21 October 2019


Basic Insert to express Cas13a (Lbu) and a guideRNA array in S. cerevisiae

This part enables the expression of Cas13a (Lbu) and a guide RNA array containing seven guide RNAs that target essential genes in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4539
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4564
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 788
    Illegal BglII site found at 1724
    Illegal BglII site found at 1988
    Illegal BglII site found at 2492
    Illegal BglII site found at 2978
    Illegal BglII site found at 3086
    Illegal BglII site found at 3416
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4539
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4539
    Illegal AgeI site found at 55
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3097