Difference between revisions of "Part:BBa K3081112"

Revision as of 11:30, 21 October 2019

a 20bp sequence targeting the R4(bottom strand) DnaA box on E.coli oriC

The 20 bp sgRNA targets to the R4- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R4 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R4 box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]

Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 <br> The 20 bp sgRNA targets to the R4- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R4 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R4 box, severe arrest and inhibition of genome replication initiation is achieved. For more detailed information, see <partinfo>BBa_K3081058</partinfo>
+<center>
+https://2019.igem.org/wiki/images/0/0a/T--Peking--box.png
+</center>
+<center>
+Figure1: Different boxes related to <i>E.coli</i> genome replication.This figure is adapted from the review of Costa, A. et al[1]
+</center>
+Reference:
+[1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.
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