Difference between revisions of "Part:BBa K3098008"

Revision as of 11:29, 21 October 2019

GST+PgD5

In order to purify the PgD5 produced by E.Coli and increase its solubility, a GST tag is added at the N-terminal of the sequence . GST tag can help the peptides dissolve in cytoplasm to help the expression of the peptide.During purification, GST tag can bind with the corresponding column to keep the target protein when the impurities leave. After washing, PreScission Protease (also have GST tag) will be added to cut the GST tag from the target protein, in this way, we'll be able to collect pure solution of the target protein without adding any more impurities.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 688
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 784
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 85

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3098008 short</partinfo>
-In order to purify the PgD5 produced by E.Coli and increase its solubility, a GST tag is added at the N-terminal of the sequence, it can help the peptide dissolve in cytoplasm to help the expression of the peptide.
+In order to purify the PgD5 produced by E.Coli and increase its solubility, a GST tag is added at the N-terminal of the sequence
+.
-<During purification, GST tag can bind with the corresponding column to keep the target protein when the impurities leave. After washing, PreScission Protease will be added to cut the GST tag from the target protein
+GST tag can help the peptides dissolve in cytoplasm to help the expression of the peptide.During purification, GST tag can bind with the corresponding column to keep the target protein when the impurities leave. After washing, PreScission Protease (also have GST tag) will be added to cut the GST tag from the target protein, in this way, we'll be able to collect pure solution of the target protein without adding any more impurities.
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