Difference between revisions of "Part:BBa K2967011"
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<partinfo>BBa_K2967011 short</partinfo> | <partinfo>BBa_K2967011 short</partinfo> | ||
| − | It is the sequence of inducible promoter P''hrpL''. HrpR([https://parts.igem.org/Part:BBa_K2967008 | + | It is the sequence of inducible promoter P''hrpL''. HrpR([https://parts.igem.org/Part:BBa_K2967008 BBa_K2967008]) protein binds to HrpS([https://parts.igem.org/Part:BBa_K2967009 BBa_K2967009]) protein forming a complex and then triggering the transcription of induced promoter P''hrpL''. It functions like a AND logic gate to open an gene circuit. |
The hrpL promoter (P''hrpL'') contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays. | The hrpL promoter (P''hrpL'') contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays. | ||
Latest revision as of 11:04, 21 October 2019
The inducible promoter, PhrpL
It is the sequence of inducible promoter PhrpL. HrpR(BBa_K2967008) protein binds to HrpS(BBa_K2967009) protein forming a complex and then triggering the transcription of induced promoter PhrpL. It functions like a AND logic gate to open an gene circuit. The hrpL promoter (PhrpL) contains a functional UAS between positions −147 to −36 relative to the transcription start-site, to which purified HrpR, HrpS and HrpRS bind in electrophoretic mobility shift assays.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]