Difference between revisions of "Part:BBa K3110006"
 
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<partinfo>BBa_K3110006 short</partinfo>
 
<partinfo>BBa_K3110006 short</partinfo>
  
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This construct has L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a weak RBS.
 
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lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various  lower and higher  concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
 
  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<partinfo>BBa_K3110006 parameters</partinfo>
 
<partinfo>BBa_K3110006 parameters</partinfo>
 
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<h1>Usage and Biology</h1>
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 +
This construct has L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a weak RBS.
 +
lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various  lower and higher  concentrations of lldD by varying  the strength of the promoter and RBS controlling its production.
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<h1>Characterization</h1>
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Due to time constraint, we couldn't characterize the construct.

Latest revision as of 11:00, 21 October 2019


Strong Promoter Weak RBS lldD


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1201
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 638


Usage and Biology

This construct has L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a weak RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower and higher concentrations of lldD by varying the strength of the promoter and RBS controlling its production.

Characterization

Due to time constraint, we couldn't characterize the construct.