Difference between revisions of "Part:BBa K3110004"
 
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<h1>Characterization</h1>
 
<h1>Characterization</h1>
  
−
The SOEing of lldR and lldD was done. But, due to shortage of time we couldn't make this construct and characterize it.
+
Due to shortage of time we couldn't characterize it.

Latest revision as of 10:56, 21 October 2019


Strong Promoter Strong RBS lldD Terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1202
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 639


Usage and Biology

L-Lactate Dehydrogenase (lldD) under the control of a strong promoter and a strong RBS. lldD cleaves L-lactate to pyruvate. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various lower concentrations of lldD by varying the strength of the Promoter and RBS controlling its production.

Characterization

Due to shortage of time we couldn't characterize it.