Difference between revisions of "Part:BBa K108030:Design"
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===Source=== | ===Source=== | ||
| − | LacI and T7 terminator was cloned from pET-15b(Novagen). | + | LacI operator, LacI and T7 terminator was cloned from pET-15b(Novagen). |
===References=== | ===References=== | ||
Revision as of 06:01, 25 October 2008
TARS-LacI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 31
Design Notes
TARS sequence was designed according to reference 1.
LVA sequence was designed according to reference 2.
PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.
Source
LacI operator, LacI and T7 terminator was cloned from pET-15b(Novagen).
References
1. Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS. 2004, 68(3):474–500.
2. An Artifical Aptazyme-Based Riboswitch and its Cascading System in E.coli. ChemBioChem. 2008, 9:206-209.
3. New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 1998, 64(6):2240–2246.