Difference between revisions of "Part:BBa K3117024:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
...
+
Our flexible linker is composed of small, non-polar (e.g. Gly) and polar (e.g. Ser or Thr) amino acids. By adjusting the copy number, in this case 4 repeats of GGGGS, the length of this GS linker can be optimized to achieve appropriate separation of the proteins of interest [1]. 3 repeats ensure a high level of flexibility.
−
 
+
  
  
 
===Source===
 
===Source===
  
−
...
+
This part comes from genomic DNA.
  
 
===References===
 
===References===
 +
 +
[1]: Chen, Xiaoying; Zaro, Jennica L.; Shen, Wei-Chiang (2013): Fusion protein linkers: property, design and functionality. In: Advanced drug delivery reviews 65

Latest revision as of 10:45, 21 October 2019


(Glycine)x4-Serine Linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our flexible linker is composed of small, non-polar (e.g. Gly) and polar (e.g. Ser or Thr) amino acids. By adjusting the copy number, in this case 4 repeats of GGGGS, the length of this GS linker can be optimized to achieve appropriate separation of the proteins of interest [1]. 3 repeats ensure a high level of flexibility.


Source

This part comes from genomic DNA.

References

[1]: Chen, Xiaoying; Zaro, Jennica L.; Shen, Wei-Chiang (2013): Fusion protein linkers: property, design and functionality. In: Advanced drug delivery reviews 65