Difference between revisions of "Part:BBa K108012:Design"

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===References===
 
===References===
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1. NCBI.

Latest revision as of 05:51, 25 October 2008

MotA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 323
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 61
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 829


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

MotA was cloned from E.coli K12 DH10B genome (reference 1).

References

1. NCBI.