Difference between revisions of "Part:BBa K108008:Design"

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===References===
 
===References===
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1. NCBI.

Latest revision as of 05:23, 25 October 2008

FliI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1008
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 415
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.


Source

FliI was cloned from E.coli K12 DH10B genome (reference 1).

References

1. NCBI.