Difference between revisions of "Part:BBa K3286216"
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The following composite parts contains the cyanobacterial circadian oscillator core genes, expressed in an operon driven by the rhamnose-inducible rhaBAD promoter (PrhaBAD) [1]. The core circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942 is composed of three components, KaiA, KaiB, and KaiC, expressed as monocistronic kaiA and dicistronic kaiBC transcripts. The kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate a circadian oscillator. KaiB can bind to KaiA, therefore opposing KaiA’s stimulatory activity by sequestering it, and to KaiC, driving the KaiC phosphorylation cycle in a clockwise direction. | The following composite parts contains the cyanobacterial circadian oscillator core genes, expressed in an operon driven by the rhamnose-inducible rhaBAD promoter (PrhaBAD) [1]. The core circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942 is composed of three components, KaiA, KaiB, and KaiC, expressed as monocistronic kaiA and dicistronic kaiBC transcripts. The kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate a circadian oscillator. KaiB can bind to KaiA, therefore opposing KaiA’s stimulatory activity by sequestering it, and to KaiC, driving the KaiC phosphorylation cycle in a clockwise direction. | ||
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Latest revision as of 10:24, 21 October 2019
rhamnose-inducible rhaBAD promoter + kaiABC Operon
The following composite parts contains the cyanobacterial circadian oscillator core genes, expressed in an operon driven by the rhamnose-inducible rhaBAD promoter (PrhaBAD) [1]. The core circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942 is composed of three components, KaiA, KaiB, and KaiC, expressed as monocistronic kaiA and dicistronic kaiBC transcripts. The kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate a circadian oscillator. KaiB can bind to KaiA, therefore opposing KaiA’s stimulatory activity by sequestering it, and to KaiC, driving the KaiC phosphorylation cycle in a clockwise direction.
- Chen, AH., Lubkowicz, D., Yeong, V., Chang, RL., and Silver, PA. (2015) Transplantability of a circadian clock to a noncircadian organism. Sci. Adv. 1, e1500358.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 559
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1307
Illegal BamHI site found at 2220
Illegal XhoI site found at 2287 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2943
Illegal AgeI site found at 1039 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2350