Difference between revisions of "Part:BBa K3171171"
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V. natriegens has been reported to have a remarkable doubling time of 9.8 min. As is the case for the better-studied but slower-growing E. coli, V. natriegens increases its number of ribosomes with the growth rate in order to achieve its extraordinarily high rate of protein synthesis. Multiple mechanisms contribute to this high ribosome synthesis efficiency, including high rRNA gene copy number; strong promoters that contain near-consensus −10, −35, and UP elements; and activation by the transcription factor Fis. In addition, V. natriegens rRNA promoters exhibit the relatively short-lived open-complex characteristic of rRNA promoters in E. coli, potentially contributing to the regulation of these promoters in vivo. The native promoter P1 is constitutive. | V. natriegens has been reported to have a remarkable doubling time of 9.8 min. As is the case for the better-studied but slower-growing E. coli, V. natriegens increases its number of ribosomes with the growth rate in order to achieve its extraordinarily high rate of protein synthesis. Multiple mechanisms contribute to this high ribosome synthesis efficiency, including high rRNA gene copy number; strong promoters that contain near-consensus −10, −35, and UP elements; and activation by the transcription factor Fis. In addition, V. natriegens rRNA promoters exhibit the relatively short-lived open-complex characteristic of rRNA promoters in E. coli, potentially contributing to the regulation of these promoters in vivo. The native promoter P1 is constitutive. | ||
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| + | Genes under the control of this promoter are constitutively transcribed by the organism’s ribosomal protein P1. This part was obtained by the sequencing offer of gBLOCKS from IDT. We show that there is a 225 times increase of fluorescence using the P1 promoter in E. coli compared to E. coli without a plasmid. At the same time, measurement in V. natriegens showed a 3 times increase of fluorescence (figure 1). | ||
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Revision as of 09:55, 21 October 2019
Vibrio natriegens native P1 promoter
V. natriegens has been reported to have a remarkable doubling time of 9.8 min. As is the case for the better-studied but slower-growing E. coli, V. natriegens increases its number of ribosomes with the growth rate in order to achieve its extraordinarily high rate of protein synthesis. Multiple mechanisms contribute to this high ribosome synthesis efficiency, including high rRNA gene copy number; strong promoters that contain near-consensus −10, −35, and UP elements; and activation by the transcription factor Fis. In addition, V. natriegens rRNA promoters exhibit the relatively short-lived open-complex characteristic of rRNA promoters in E. coli, potentially contributing to the regulation of these promoters in vivo. The native promoter P1 is constitutive.
Genes under the control of this promoter are constitutively transcribed by the organism’s ribosomal protein P1. This part was obtained by the sequencing offer of gBLOCKS from IDT. We show that there is a 225 times increase of fluorescence using the P1 promoter in E. coli compared to E. coli without a plasmid. At the same time, measurement in V. natriegens showed a 3 times increase of fluorescence (figure 1).