Difference between revisions of "Part:BBa K2926001"
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− | + | This part codes for Cas13a derived from <i>Leptotrichia buccalis</i>. The natural EcoRI and PstI restriction sites were removed via mutagenesis PCR. | |
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Revision as of 09:32, 21 October 2019
Cas13a Lbu
This part codes for Cas13a derived from Leptotrichia buccalis. The natural EcoRI and PstI restriction sites were removed via mutagenesis PCR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 344
Illegal BglII site found at 1280
Illegal BglII site found at 1544
Illegal BglII site found at 2048
Illegal BglII site found at 2534
Illegal BglII site found at 2642
Illegal BglII site found at 2972 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2653