Difference between revisions of "Part:BBa K3286215"
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The folowwing composite parts contains the cyanobacterial circadian oscillator core genes. The core circadian clock of the cyanobacterium <em>Synechococcus elongatus</em> PCC 7942 is composed of three components, KaiA, KaiB, and KaiC, expressed as monocistronic kaiA and dicistronic kaiBC transcripts [1]. The kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate a circadian oscillator. KaiB can bind to KaiA, therefore opposing KaiA’s stimulatory activity by sequestering it, and to KaiC, driving the KaiC phosphorylation cycle in a clockwise direction.
 
The folowwing composite parts contains the cyanobacterial circadian oscillator core genes. The core circadian clock of the cyanobacterium <em>Synechococcus elongatus</em> PCC 7942 is composed of three components, KaiA, KaiB, and KaiC, expressed as monocistronic kaiA and dicistronic kaiBC transcripts [1]. The kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate a circadian oscillator. KaiB can bind to KaiA, therefore opposing KaiA’s stimulatory activity by sequestering it, and to KaiC, driving the KaiC phosphorylation cycle in a clockwise direction.
 
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Revision as of 09:21, 21 October 2019

kaiABC Operon

The folowwing composite parts contains the cyanobacterial circadian oscillator core genes. The core circadian clock of the cyanobacterium Synechococcus elongatus PCC 7942 is composed of three components, KaiA, KaiB, and KaiC, expressed as monocistronic kaiA and dicistronic kaiBC transcripts [1]. The kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate a circadian oscillator. KaiB can bind to KaiA, therefore opposing KaiA’s stimulatory activity by sequestering it, and to KaiC, driving the KaiC phosphorylation cycle in a clockwise direction.

  1. [1]Cohen, SE., and Golden, SS. (2015) Circadian Rhythms in Cyanobacteria. Microbiol. Biol. Rev. 79(4), 373-385.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1922
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1922
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1922
    Illegal BglII site found at 288
    Illegal BglII site found at 3362
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1922
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1922
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
    Illegal NgoMIV site found at 818
    Illegal NgoMIV site found at 909
    Illegal NgoMIV site found at 1736
    Illegal NgoMIV site found at 1831
    Illegal NgoMIV site found at 2890
  • 1000
    COMPATIBLE WITH RFC[1000]