Difference between revisions of "Part:BBa K3137002"

Revision as of 08:45, 21 October 2019

PputA

PputA is the inducible promoter.We screened the whole-genome transcriptome data by setting the conditions that E5_FPKM>E0_FPKM*10 and E0_FPKM < 150, and selected the significant differentially expression genes which express during the early stage of bacteriophage invasion, such as dadA and lldP. We selected the promoter of PputA as our phage-induced promoter in the early stage because the PputA has the maximun value of log2（E5_FPKM/E0_FPKM）,which means the PputA has the most significant differentially expression during the early period.

Usage and Biology

PputA is the inducible promoter that When E.coli is infected by a phage, both report and respond gene circuits ((BBa_K3137013)(BBa_K3137014)) are induced to express at the same time. When a phage infects Escherichia coli into the incubation period (about 5 min), PputA will induce the bacteria to express resistant proteins((BBa_K3137006)(BBa_K3137005)) and display green fluorescence.

Characterization of PputA

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3137002 short</partinfo>
-PputA is the inducible promoter.We screened the whole-genome transcriptome data by setting the conditions that E5_FPKM>E0_FPKM*10 and E0_FPKM < 150, and selected the significant differentially expression genes which express during the early stage of bacteriophage invasion, such as dadA and lldP. We selected the promoter of PputA as our phage-induced promoter in the early stage because the PputA has the maximun value of log2（E5_FPKM/E0_FPKM）,which means the PputA has the most significant differentially expression during the early period.
+PputA is the inducible promoter.We screened the whole-genome transcriptome data by setting the conditions that E5_FPKM>E0_FPKM*10 and E0_FPKM < 150, and selected the significant differentially expression genes which express during the early stage of bacteriophage invasion, such as <i>dadA</i> and <i>lldP</i>. We selected the promoter of PputA as our phage-induced promoter in the early stage because the PputA has the maximun value of log2（E5_FPKM/E0_FPKM）,which means the PputA has the most significant differentially expression during the early period.
   [[Image:EARLY PROMOTER 2.gif|400X400 px]]
@@ Line 9: / Line 9: @@
 ===Usage and Biology===
-PputA is the inducible promoter that When E.coli is infected by a phage, both report and respond gene circuits (([https://parts.igem.org/Part:BBa_K3137013# BBa_K3137013])([https://parts.igem.org/Part:BBa_K3137014# BBa_K3137014])) are induced to express at the same time. When a phage infects Escherichia coli into the incubation period (about 5 min), PputA will induce the bacteria to express resistant proteins(([https://parts.igem.org/Part:BBa_K3137006# BBa_K3137006])([https://parts.igem.org/Part:BBa_K3137005# BBa_K3137005])) and display green fluorescence.
+PputA is the inducible promoter that When <i>E.coli</i> is infected by a phage, both report and respond gene circuits (([https://parts.igem.org/Part:BBa_K3137013# BBa_K3137013])([https://parts.igem.org/Part:BBa_K3137014# BBa_K3137014])) are induced to express at the same time. When a phage infects <i>Escherichia coli</i> into the incubation period (about 5 min), PputA will induce the bacteria to express resistant proteins(([https://parts.igem.org/Part:BBa_K3137006# BBa_K3137006])([https://parts.igem.org/Part:BBa_K3137005# BBa_K3137005])) and display green fluorescence.
 [[Image:EARLY PROMOTER.png|400px|left]]