Difference between revisions of "Part:BBa K3113102"
Theresakeil (Talk | contribs) |
|||
| Line 5: | Line 5: | ||
This sequence codes for the loop structure coiled-coil 9 which interacts with coiled-coil 10 and allows us to specifically load proteins and RNA into vesicles. | This sequence codes for the loop structure coiled-coil 9 which interacts with coiled-coil 10 and allows us to specifically load proteins and RNA into vesicles. | ||
| − | < | + | <h2>Usage</h2> |
| − | + | ||
| + | To increase the modularity of our system we decided to test coiled-coil proteins. These coiled coils mimic the interactions of DNA helix-helix interactions. The coiled-coil protein interaction allows the loading of more than one protein and larger proteins. We are using these helical structures to load RNA binding proteins into exosomes or virus-like-particles. | ||
| + | |||
| + | <h2>Biology</h2> | ||
| + | |||
| + | We describe a system analogous to designed DNA nanostructures in which protein coiled-coil (CC) dimers serve as building blocks for modular de novo design of polyhedral protein cages that efficiently self-assemble in vitro and in vivo.<ref>Ljubetič, A., et al. (2017). "Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo." Nature Biotechnology 35: 1094.</ref> | ||
| + | |||
| + | |||
| + | <h2>Characterization</h2> | ||
| + | |||
| + | |||
<!-- --> | <!-- --> | ||
| Line 12: | Line 22: | ||
<partinfo>BBa_K3113102 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3113102 SequenceAndFeatures</partinfo> | ||
| + | <h2>References</h2> | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 08:40, 21 October 2019
P9SN
This sequence codes for the loop structure coiled-coil 9 which interacts with coiled-coil 10 and allows us to specifically load proteins and RNA into vesicles.
Usage
To increase the modularity of our system we decided to test coiled-coil proteins. These coiled coils mimic the interactions of DNA helix-helix interactions. The coiled-coil protein interaction allows the loading of more than one protein and larger proteins. We are using these helical structures to load RNA binding proteins into exosomes or virus-like-particles.
Biology
We describe a system analogous to designed DNA nanostructures in which protein coiled-coil (CC) dimers serve as building blocks for modular de novo design of polyhedral protein cages that efficiently self-assemble in vitro and in vivo.[1]
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Ljubetič, A., et al. (2017). "Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo." Nature Biotechnology 35: 1094.