Difference between revisions of "Part:BBa K3152005"
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We contructed it successfully and took out two red colonies from the LB solid medium and digested by EcoRI and PstI restriction enzymes to further verify the correctness of the connection. | We contructed it successfully and took out two red colonies from the LB solid medium and digested by EcoRI and PstI restriction enzymes to further verify the correctness of the connection. | ||
| − | [[File:Antibiotics-R1.png|300px|thumb| | + | [[File:Antibiotics-R1.png|300px|thumb|center|Figure 1 Photos of transformants of “pSB1C3-mec operator-mCherry” on LB agar broth.]] |
Latest revision as of 08:28, 21 October 2019
mec operator-mCherry
This composite part is a report pathway. The mec operator is responsible for mCherry (BBa_J06504) expressing.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
We contructed it successfully and took out two red colonies from the LB solid medium and digested by EcoRI and PstI restriction enzymes to further verify the correctness of the connection.
