Difference between revisions of "Part:BBa K3311005"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This composite part was improved based on the suggestions from QHFZ-China 2019 iGEM team during an online meet up. The leader of their team told us that the pHucO we were using is too weak to let the gene pathway to produce enough mCherry. Plus, the promoter of HucR is too strong, causing a heaving repression on the transcription on mcherry. Furthermore, they provided two articles to support their | + | This composite part was improved based on the suggestions from QHFZ-China 2019 iGEM team during an online meet up. The leader of their team told us that the pHucO we were using is too weak to let the gene pathway to produce enough mCherry. Plus, the promoter of HucR is too strong, causing a heaving repression on the transcription on mcherry. Furthermore, they provided two articles to support their suggestion[1,2]. According to their suggestion, we designed an improved plan: we added a strong constitutive promoter (BBa_J23100) in front of pHucO, enhancing the transcription of mcherry. In addition, the original strong promoter before HucR is replaced by a weaker constitutive promoter (BBa_J23110) so as to weaken the repression on pHucO. |
| + | '''Reference''' | ||
| + | |||
| + | 1. Kemmer, C., Gitzinger, M., Daoud-El Baba, M., Djonov, V., Stelling, J., and Fussenegger, M. (2010) Self-sufficient control of urate homeostasis in mice by a synthetic circuit, Nature biotechnology 28, 355-360. | ||
| + | |||
| + | 2. Wan, X., Volpetti, F., Petrova, E., French, C., Maerkl, S. J., and Wang, B. (2019) Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals, Nature chemical biology 15, 540-548. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3311005 parameters</partinfo> | <partinfo>BBa_K3311005 parameters</partinfo> | ||
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Latest revision as of 08:13, 21 October 2019
Improved HucR-pHucO-mCherry
It is an improvement on BBa_K3311004. We added a strong constitutive promoter (BBa_J23100) in front of pHucO, enhancing the transcription of mCherry. In addition, the original strong promoter before HucR is replaced by a weaker constitutive promoter (BBa_J23110) so as to weaken the repression on pHucO.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 759
Illegal NheI site found at 782 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This composite part was improved based on the suggestions from QHFZ-China 2019 iGEM team during an online meet up. The leader of their team told us that the pHucO we were using is too weak to let the gene pathway to produce enough mCherry. Plus, the promoter of HucR is too strong, causing a heaving repression on the transcription on mcherry. Furthermore, they provided two articles to support their suggestion[1,2]. According to their suggestion, we designed an improved plan: we added a strong constitutive promoter (BBa_J23100) in front of pHucO, enhancing the transcription of mcherry. In addition, the original strong promoter before HucR is replaced by a weaker constitutive promoter (BBa_J23110) so as to weaken the repression on pHucO.
Reference
1. Kemmer, C., Gitzinger, M., Daoud-El Baba, M., Djonov, V., Stelling, J., and Fussenegger, M. (2010) Self-sufficient control of urate homeostasis in mice by a synthetic circuit, Nature biotechnology 28, 355-360.
2. Wan, X., Volpetti, F., Petrova, E., French, C., Maerkl, S. J., and Wang, B. (2019) Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals, Nature chemical biology 15, 540-548.