Difference between revisions of "Part:BBa K3311004"
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As for “HucR-pHucO-mCherry” (Figure 2), while uric acid is absent, HucR will bind to pHucO operator, inhibiting the transcription of downstream genes--mCherry. In this situation, the color of the sample will not change. While uric acid is present, it will change the structure of HucR, causing it to leave pHucO, allowing mCherry to transcript, the sample will turn red.
 
As for “HucR-pHucO-mCherry” (Figure 2), while uric acid is absent, HucR will bind to pHucO operator, inhibiting the transcription of downstream genes--mCherry. In this situation, the color of the sample will not change. While uric acid is present, it will change the structure of HucR, causing it to leave pHucO, allowing mCherry to transcript, the sample will turn red.
 
Now, we successfully constructed the plasmid “HucR-pHucO-mCherry”（Figure1-2）.
 
Now, we successfully constructed the plasmid “HucR-pHucO-mCherry”（Figure1-2）.
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[[File:HucR-pHucO_1.png|300px|thumb|left|Figure 1 Photos of transformants of pSB1C3-HucR-pHucO-mCherry on LB agar broth.]]  
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[[File:HucR-pHucO_1.png|400px|thumb|center|Figure 1 Photos of transformants of pSB1C3-HucR-pHucO-mCherry on LB agar broth.]]
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[[File:HucR-pHucO_3.png|300px|thumb|left|Figure 2 Enzyme digestion analysis pSB1C3-HucR-pHucO-mCherry.]]
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[[File:HucR-pHucO_3.png|400px|thumb|center|Figure 2 Enzyme digestion analysis pSB1C3-HucR-pHucO-mCherry.]]
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Revision as of 08:09, 21 October 2019


HucR-pHucO-mCherry

The “HucR-pHucO-mCherry” is composed of “Anderson Strong-RBS-HucR”(BBa_K3311003) and “pHucO-mCherry”(BBa_K3311002 ). It is a important tool for our part function test. HucR is promoted by a constitutive promoter, while the transcription of mCherry is controlled by pHucO.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

As for “HucR-pHucO-mCherry” (Figure 2), while uric acid is absent, HucR will bind to pHucO operator, inhibiting the transcription of downstream genes--mCherry. In this situation, the color of the sample will not change. While uric acid is present, it will change the structure of HucR, causing it to leave pHucO, allowing mCherry to transcript, the sample will turn red. Now, we successfully constructed the plasmid “HucR-pHucO-mCherry”（Figure1-2）.

Figure 1 Photos of transformants of pSB1C3-HucR-pHucO-mCherry on LB agar broth.
Figure 2 Enzyme digestion analysis pSB1C3-HucR-pHucO-mCherry.